The Chip is loaded into the MassARRAY Analyzer 4 MALDI-TOF mass spectrometer, where the analyte/matrix co-crystals are desorbed and ionized. The analyte molecules are accelerated and their time-of-ﬂight is measured. The MassARRAY Analyzer 4 is designed to detect DNA within a mass range of approximately 4,500 Da to 9,000 Da, and can distinguish between analytes separated by 16 Da or more. (Analytes are typically 18-25 nucleotides.) Peak area ratios provide a quantitative readout.
The MassARRAY System has the ability to automatically process up to two multiplexed 24-, 96-, or 384-pad SpectroCHIP Arrays per run, and comes with automated sample handling instruments based on the needs of your laboratory.