Microbiological Testing-Nissui 2017-12-13T12:17:45+00:00

microbiological testing

NISSUI

  • Quick and easy to determine. Accurate counting.
  • No need of complicated dilution and time-consuming steps.
  • Small and delicate dry medium is convenient for storage and culturing.
  • Small scale of sample can diffuse automatically.
  • Compact dry can be kept in room temperature for one year.
  • It is easy to pick colonies according to the color change of the medium.

Total Count

  • Since tetrazolium salt is added in the medium, the colonies will be red and is easy to determine.
  • Steps:
    1. 35 ± 2 ℃ incubation for 48 hr
    2. Count red colonies

EC ( E. coli, Coliform)

  • E. coli and Coliform are presented in deferent colors according to the substrate of color presenting enzyme, Magenta-GAL and X-Gluc. It is easy to determine the colonies of E.coli or coliform by colors.
  • Steps:
    1. 35 ± 2 ℃ incubation for 24 hr
    2. Count blue colonies as E. coli and total colonies as coliform.

CF (Coliform)

  • E. coli including E. coli O157 are presented in blue by adding X-GAL, which is the substrate of color presenting enzyme, into the medium.
  • Gram negative bacteria other than E. coli are presented in white.
  • Steps:
    1. 35 ± 2 ℃ incubation for 24 hr
    2. Count blue colonies as coliform.

X-SA (Staphylococcus aureus)

  • Staphylococcus aureus are presented in light blue or blue according to the substrate for color presenting enzyme.
  • The bacteria other than Staphylococcus aureus are presented in white.
  • Steps:
    1. 35 ± 2 ℃ incubation for 24 ± 2 hr
    2. Count light blue or blue colonies as SA.

VP (Vibrio parahaemolyticus)

  • Reformed according to TCBS agar. Inhibit the growth of bacteria other than Vibrio.
  • V. parahaemolyticus are presented in blue according to the substrate of color presenting enzyme. Bacteria other than VP are presented in white.
  • Steps:
    1. 35 ± 2 ℃ incubation for 18-20 hr
    2. Count blue colonies as V. parahaemolyticus

YM ( Yeast and Mold)

  • The antibiotics in the medium inhibits the growth of bacteria.
  • Most of the yeast presented in blue. Mold is black and cotton-like.
  • Steps:
    1. 20 ~ 25 ℃ incubation for 3 ~ 7 days
    2. Count blue or white colonies as yeast and black cotton-like colonies as mold.

YMR ( Yeast and Mold rapid detection)

  • The antibiotics in the medium inhibits the growth of bacteria.
  • Most of the yeast presented in blue. Mold is black and cotton-like.
  • Steps:
    1. 20 ~ 25 ℃ incubation for 2 ~ 3 days
    2. Count blue or white colonies as yeast and black cotton-like colonies as mold.

X-BC (Bacillus cereus)

  • Other bacteria will not grow on the medium except Bacillus cereus.
  • The colonies of Bacillus cereus will be green or blue.
  • Steps:
    1. Incubate in 35 ± 2 ℃ for 24± 2 hr
    2. Count the green or blue colonies as Bacillus cereus.

SL (Salmonella)

  • The lysine decarboxylase of Salmonella will increase the pH of the medium and change the color of the medium from purple to yellow.
  • The colonies are presented in green by the substrate of the color presenting enzyme.
  • Salmonella expresses H2S, which will change the color of colonies into black.
  • Steps:
    1. Pre-incubation in 35 ~ 37℃ for 20 ~ 24 hr.
    2. Incubate on compact dry in 41 ~ 43℃ for 20 ~ 24 hr.
    3. Count the colonies of Salmonella according to the color of medium and colonies.

PA (Pseudomonas aeruginosa)

  • The antibiotics in the medium inhibits the growth of bacteria.
  • Most of the yeast presented in blue. Mold is black and cotton-like.
  • Steps:
    1. 34 ~ 36 ℃ incubation for 45 ~ 51 hours
    2. Count blue or white colonies as yeast and black cotton-like colonies as mold.

Microbes Detection Kit & Bacterialogical Testing Medium for Food and Environment

Microbes’ Incubation Medium and Reagents

Download Datasheet:

Compact Dry v1
Compact Dry v2
Compact Dry-EC
Compact Dry-TC
Compact Dry-X-SA
Compact Dry-YM