The experiment will begin with the fragmentation of the target sequence, and ligate with the sequencing index to set up the sequence library. Then use the NGS system to amplify the sequence library, and at the mean time perform the sequencing detection through the synthesis process. The process simultaneously identifies DNA bases while incorporating them into a nucleic acid chain. Each base emits a unique fluorescent signal as it is added to the growing strand, which is used to determine the order of the DNA sequence. NGS technology can be used in several genomic research such as targeting gene sequencing, RNA sequencing, and metagenomics, and so on.